Tagged: Restriction endonucleases

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A clone is a population of identical organisms derived from a single parental organism. In Molecular biology- A clone is a collection of molecules or cells, all identical to an original molecule or cell. If the original cells harbored a recombinant DNA molecule in the form of a plasmid, the plasmids within the millions of cells in a bacterial colony represent a clone of original DNA molecules; these molecules can be isolated and studied. The mechanism of cloning involves , i) Cutting DNA at precise locations, ii) Joining two DNA fragments covalently, iii) Selection of a small molecule of DNA...

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Restriction enzymes and DNA fingerprinting

DNA fingerprinting is used in the legal world to determine parentage, genealogy, or other genetic relationships, or to implicate suspects in law enforcement investigations involving violent crimes. Fragments produced by various restriction enzymes from a number of different loci can be used to identify individuals with the accuracy of a fingerprint. Therefore, this technique is called “Fingerprinting”. Which “one” of the following statements is incorrect about restriction enzymes? A. They are site specific endonucleases B.  The restriction sites are palindrome in nature C. All restriction enzymes are of bacterial origin D. They always produce complementary single stranded overhangs E. They...

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